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Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Updated: Mar 19, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Construct design for precise DNA insertion in plants.

Adnan Muzaffar¹, Md Torikul Islam¹, Adam M Guss¹

¹Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; The Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

Trends in Plant Science

|March 18, 2026

Summary

This summary is machine-generated.

This review details plant genome engineering via targeted DNA insertion (knock-in). It offers practical guidance on construct design and assesses methods for precise, efficient crop improvement with minimal off-target effects.

Keywords:

DNA construct genome editing high-precision engineering knock-in plant biotechnology synthetic biology

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Area of Science:

Plant genomics and synthetic biology
Agricultural biotechnology and crop improvement

Background:

Precise DNA insertion into plant genomes is crucial for genetic research and crop enhancement.
Limited practical guidance exists for designing constructs for targeted DNA insertion (knock-in) in plants.

Purpose of the Study:

To provide an in-depth review of construct design principles for plant knock-in strategies.
To assess current knock-in methods for various insertion types (short, large, multifragment) and their limitations.
To explore novel nonplant technologies for improving plant knock-in efficiency and precision.

Main Methods:

Literature review of existing plant knock-in technologies and construct design principles.
Comparative analysis of strengths, limitations, and requirements of different knock-in methods.
Exploration of advanced technologies from nonplant systems for potential application in plants.

Main Results:

Current knock-in methods have varying efficiencies and limitations depending on insertion size and complexity.
Specific construct design strategies are essential for successful targeted DNA insertion.
Advanced technologies show promise for enhancing knock-in precision and efficiency in plants.

Conclusions:

Effective construct design is key to successful targeted DNA insertion in plants.
A comprehensive understanding of knock-in methods is needed for efficient crop improvement.
Adopting advanced technologies can overcome current limitations in plant genome engineering.