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Updated: Mar 19, 2026

Author Spotlight: Establishing an Accurate Microhardness Testing Protocol for Craniofacial Tissues
Published on: April 26, 2024
Assessment of Microhardness on Root Canal Dentin Using Chelating Agents: A Comparative Study
Anandamoy Bagchi1, Kapil R Jadhav2, Chitharanjan Shetty3
1Department of Pediatric and Preventive Dentistry, Kalinga Institute of Dental Sciences (KIDS), Campus 5, KIIT (DU), Patia, Bhubaneswar, Odisha, India.
Background:
The effect of chelating agents on root canal dentin microhardness is a significant factor in endodontic treatment outcomes. Comparative evaluation of their impact is essential for clinical optimization.
Objective:
To assess the microhardness of root canal dentin treated with different chelating agents.
Methods:
Thirty extracted human premolars were divided into three groups (each n = 10), based on the chelating agent used: Group 1 (EDTA 17%), Group 2 (Citric Acid 10%), and Group 3 (QMix). Microhardness was measured using a Vickers microhardness tester. Statistical analysis was performed using ANOVA and post hoc tests.
Results:
All chelating agents caused a significant reduction in dentin microhardness. EDTA showed the highest reduction, followed by Citric Acid and QMix. Statistically significant differences were observed between groups (P < 0.05).
Conclusion:
QMix was the least detrimental to dentin microhardness, suggesting its potential advantage in preserving dentin integrity during root canal preparation.
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