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Related Experiment Video

Updated: Mar 19, 2026

Author Spotlight: Development of an Efficient and Feasible Method of Retinal Pigment Epithelial Cell Freezing
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Stem cell preservation with novel cryoprotectants.

Amanda N Abraham1, Miyah N Awad1, Izabela Miłogrodzka1

  • 1RMIT University , Melbourne, Victoria, Australia.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

New cryoprotective agents (CPAs) based on deep eutectic solvents show promise for preserving stem cells. These novel CPAs offer improved recovery and reduced toxicity compared to current methods, enhancing clinical outcomes.

Keywords:
cell preservationcryopreservationcryoprotectantsdeep eutectic solventsstem cells

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Cryopreservation is essential for long-term biological sample storage.
  • Current cryoprotective agents (CPAs) like DMSO and glycerol have limitations, especially for stem cell preservation.
  • Effective stem cell preservation is critical for clinical applications.

Purpose of the Study:

  • To screen novel CPAs based on deep eutectic solvents (DESs) for toxicity and permeability.
  • To evaluate the efficacy of these new CPAs in preserving human mesenchymal stem cells (MSCs).
  • To develop improved cryopreservation protocols for stem cells.

Main Methods:

  • Screening of seven potential CPAs, derived from DESs, using standard cell lines (THP-1, HaCat).
  • Assessment of CPA toxicity and permeability.
  • Testing of promising CPAs with human mesenchymal stem cells (MSCs).

Main Results:

  • New cryoprotectants, including mixtures of proline with glycerol/ethylene glycol or alanine with glycerol, demonstrated comparable or superior recovery rates to the gold standard.
  • The tested CPAs showed potential for reduced toxicity compared to conventional agents.
  • Successful cryopreservation of MSCs was achieved with the novel CPA formulations.

Conclusions:

  • Novel CPAs based on DESs offer a promising alternative for stem cell cryopreservation.
  • These new agents can improve cell recovery and reduce toxicity, leading to better clinical outcomes.
  • This study presents a new method for stem cell preservation with potential clinical impact.