Related Experiment Video
Updated: Mar 20, 2026

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
Published on: June 28, 2019
GLASS2: An Updated Database for Comprehensive Experimentally Validated GPCR-ligand Associations
Fanding Xu1, Xinzhe Zheng2, Wenkang Wang3
1Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; School of Computing, National University of Singapore, Singapore 117417, Singapore.
Abstract:
G protein-coupled receptors (GPCRs) represent one of the most important drug target families, yet comprehensive and standardized GPCR-ligand interaction data remain fragmented across multiple resources. Here, we present GLASS2, a substantially expanded and methodologically enhanced database of GPCR-ligand associations. By integrating data from major pharmacological databases with a large language model (LLM) powered text-mining pipeline applied to PubMed literature, GLASS2 delivers a 72% increase in total associations over its predecessor, encompassing 1,715 receptors and 450,736 ligands across more than one million unique interactions. Critically, GLASS2 provides task-ready classification and regression datasets with clearly defined positive and negative samples, directly supporting AI-driven drug discovery applications. The database is freely accessible through an intuitive web portal at https://zhanggroup.org/GLASS/, offering seamless navigation, structure-based search, and integration with GPCR structural resources.
Related Concept Videos
G Protein-coupled Receptors
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
G Protein-coupled Receptors
G-protein Coupled Receptors
G-protein Coupled Receptors
GPCRs Regulate Adenylyl Cylase Activity
Transducer Mechanism: G Protein–Coupled Receptors
GPCRs are also called heptahelical,...

