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Publisher Correction: Microbial growth rates captured using Raman-SIP reveal a highly active subsurface biosphere fueled by serpentinization.

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Updated: Mar 20, 2026

Using Flexible Gold-Titanium Reaction Cells to Simulate Pressure-Dependent Microbial Activity in the Context of Subsurface Biomining
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Microbial growth rates captured using Raman-SIP reveal a highly active subsurface biosphere fueled by

Srishti Kashyap1,2, Tristan A Caro3,4, Alexis S Templeton5

  • 1Department of Geological Sciences, University of Colorado Boulder, Boulder, CO, USA. srishti.kashyap@colorado.edu.

Nature Communications
|March 19, 2026
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Summary

Microbial growth rates in deep serpentinite systems were measured using Raman-stable isotope probing. These findings reveal faster-than-expected microbial growth, indicating significant habitability in these unique subsurface environments.

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Area of Science:

  • Geomicrobiology
  • Subsurface Microbiology
  • Isotope Geochemistry

Background:

  • Microbial growth rates are key to understanding subsurface biogeochemical processes.
  • These rates are largely unknown in deep, rock-hosted environments like serpentinites.
  • Serpentinizing systems offer unique geochemical conditions for microbial life.

Purpose of the Study:

  • To measure single-cell microbial growth rates in serpentinite fluids.
  • To investigate the influence of fluid geochemistry on microbial growth.
  • To assess the habitability and biogeochemical dynamics of continental serpentinizing ecosystems.

Main Methods:

  • Raman-stable isotope probing with deuterated water (Raman-2H-SIP) was applied.
  • Single-cell microbial growth rates were measured in fluids from Oman serpentinites (250-270 m depth).
  • Cell-specific generation times were inferred across a range of geochemical conditions.

Main Results:

  • Observed wide distributions in microbial growth rates, varying with fluid geochemistry.
  • Inferred cell-specific microbial generation times ranged from days to years.
  • Measured generation times were generally faster than previously reported for subsurface ecosystems.
  • Bicarbonate amendment stimulated rapid growth and methane production, indicating preference for dissolved inorganic carbon.

Conclusions:

  • Actively serpentinizing systems can support extensive microbial growth.
  • Continental serpentinizing ecosystems exhibit significant habitability.
  • Cell-specific growth and methanogenesis rates provide quantitative insights into reservoir-scale biogeochemical dynamics.