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Innovative In Vitro-In Silico Platform for Dose-Response Modeling in Canine Bladder Cancer: A 3D Organoid- and

Mackenzie Long1, Yvonne Peng2,3, Saumya Gade2,3

  • 1Department of Pathology, College of Veterinary Medicine, University of Georgia, Rm 228, 501 D.W. Brooks Drive, Athens, Georgia, 30602, USA. Mackenzie.long@uga.edu.

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|March 19, 2026
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Summary
This summary is machine-generated.

Researchers developed an in vitro-in silico system using canine organoids to predict bladder cancer treatment response. This platform assesses chemoradiation efficacy, paving the way for personalized cancer therapies.

Keywords:
Canine bladder cancerCanine organoidsChemoradiationModeling

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Area of Science:

  • Oncology
  • Biotechnology
  • Computational Biology

Background:

  • Muscle-invasive bladder cancer (MIBC) treatment response is difficult to predict, impacting patient outcomes.
  • Existing predictive models for urothelial carcinoma (UC) are limited.
  • There is a need for advanced platforms to model and predict MIBC treatment efficacy.

Purpose of the Study:

  • To develop and validate an integrated in vitro-in silico system for predicting chemoradiation response in urothelial carcinoma (UC).
  • To utilize canine UC-derived organoids as a model that mirrors human MIBC.
  • To quantitatively assess the combined effects of radiation and cisplatin on cancer cell viability.

Main Methods:

  • Established canine UC-derived organoids and treated them with varying doses of radiation and cisplatin.
  • Assessed cell viability using a PrestoBlue metabolic activity assay.
  • Applied a two-dimensional Hill model within a Bayesian hierarchical framework to analyze treatment effects and variability.

Main Results:

  • Demonstrated significant inter-patient variability in response to both radiation and cisplatin.
  • Quantified varying potency for radiation (ED50: 0.55-7.6 Gy) and cisplatin (EC50: 40-174 μM).
  • Identified radiation as having a greater inhibitory contribution than cisplatin and observed synergistic effects in some cases.

Conclusions:

  • The developed in vitro-in silico system provides a robust platform for assessing chemoradiation efficacy and potency.
  • Canine UC organoids serve as a valuable preclinical model for human MIBC.
  • This approach offers potential for developing personalized and optimized chemoradiation strategies for bladder cancer.