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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Mar 20, 2026

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Published on: February 3, 2023

Machine Learning Integrates Bulk and Single-Nucleus RNA Sequence to Explore Apoptosis-Related Gene in Myocardial

Bin Li^1,2, Yuan Wu^1,2, Shude Liao²

¹Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China, rmhospital.com.

Cardiovascular Therapeutics

|March 19, 2026

Summary

Fibroblasts drive apoptosis after myocardial infarction (MI). TNFRSF12A is identified as a key regulator of apoptosis in MI, offering potential as a diagnostic biomarker and therapeutic target.

Keywords:

TNFRSF12A bioinformatics machine learning myocardial infarction

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Last Updated: Mar 20, 2026

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

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Published on: February 3, 2023

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

Area of Science:

Cardiovascular Biology
Molecular Biology
Genomics

Background:

Myocardial infarction (MI) is a leading cause of cardiovascular death.
Apoptosis is critical in cardiac remodeling post-MI but poorly understood at the single-cell level.
Bulk RNA sequencing has limitations in characterizing cellular heterogeneity in MI.

Purpose of the Study:

To elucidate the role of apoptosis in cardiac remodeling after MI at single-cell resolution.
To identify key molecular regulators of apoptosis in MI progression.
To explore the potential of identified regulators as diagnostic or therapeutic targets.

Main Methods:

Integrated multiomics data from single-nucleus RNA sequencing (snRNA-seq) and bulk RNA sequencing.
Analyzed 397 apoptosis-related genes (ARGs) using AUCell scoring and correlation analysis.
Employed Random Forest, Boruta, and LASSO algorithms to identify hub genes, validated in vitro and in vivo.

Main Results:

Single-nucleus analysis revealed significant variation in ARG activity across cardiac cells post-MI.
Fibroblasts exhibited markedly higher apoptotic activity compared to other cardiac cell types.
TNFRSF12A was identified as a key regulator of ARG activation in MI and validated in experimental models.

Conclusions:

Fibroblast-mediated apoptosis is a dominant process following MI.
TNFRSF12A is a crucial regulator in MI, demonstrating potential as a diagnostic biomarker and therapeutic target.
Multiomics integration provides novel insights into cellular mechanisms of MI.