Microprinted Epoxysilane Arrays for Conducting Microarray-Based Bioassays

A common method of fabricating microarrays requires a microarray printer, which is unavailable to most laboratories. The printing process compromises bioactivity of the printed molecules. Here, we introduced an approach to micropatterning epoxysilane, a linker traditionally used as a continuous monolayer for covalently binding of biomolecules and applied it to create a compartmentalized linker array (CLA). The results demonstrated robust covalent attachment of antibodies. Multiplexed immunoassays achieved limits of detection (LODs) at low pg/mL, outperforming standard ELISA. These findings demonstrated the versatility of this platform for low-cost, high-sensitivity bioassays, and established it as a viable method for covalently patterning of biomolecules.