Preliminary lipidomics and transcriptomics reveals stage-specific dynamic metabolic patterns from menopause...

Area of Science:

• Reproductive Endocrinology
• Metabolomics
• Genomics

Background:

• Menopause involves significant hormonal shifts, impacting women's health.
• Understanding metabolic and gene expression changes during menopausal stages is crucial.

Purpose of the Study:

• To systematically analyze clinical indicators, lipid metabolism, and leukocyte transcriptomic profiles across premenopausal, menopause transition, and postmenopausal stages.
• To explore stage-specific characteristics and associations with clinical indicators.

Main Methods:

• Collected clinical indicators and performed untargeted lipidomics and leukocyte transcriptomic sequencing.
• Utilized multivariate statistical analyses, trend clustering, volcano plots, and Venn diagrams.
• Conducted Gene Ontology (GO) and KEGG pathway enrichment analyses.

Main Results:

• Hormonal changes (decreased E2, increased FSH/LH) correlated with lipid alterations (increased CHOL, LDLC) and gene expression.
• Menopause transition showed higher BMI and significant lipid metabolic reorganization.
• Stage-specific genes enriched in inflammation and lipid metabolism pathways were identified.

Conclusions:

• Menopausal stages exhibit distinct lipid metabolism and gene expression profiles, with menopause transition being a critical phase.
• Specific lipids and genes may serve as biomarkers for menopause-associated metabolic differences.
• Findings support early warning and intervention strategies for menopause-related health issues.