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A lab-scale biofiltration system for mitigating diluted methane emissions.

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A new open-source biofiltration system effectively removes methane, a potent greenhouse gas, from diffuse sources. This modular hardware enables reproducible research and scalable solutions for methane mitigation.

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Area of Science:

  • Environmental science
  • Biotechnology
  • Chemical engineering

Background:

  • Methane (CH4) is a potent greenhouse gas with significant global warming potential.
  • Diffuse methane sources are challenging to mitigate with conventional technologies.
  • Existing biofiltration systems lack standardization, hindering reproducibility.

Purpose of the Study:

  • To develop and validate an open-source, modular lab-scale biofiltration system.
  • To enable reproducible research and facilitate adoption of biofiltration for methane mitigation.
  • To support broader applications in gas-phase bioprocessing.

Main Methods:

  • Designed and constructed a modular, lab-scale biofiltration system with triplicate packed-bed columns.
  • Integrated mass flow controllers, humidifiers, and standardized fittings for precise control and leak-free operation.
  • Validated system performance using an inlet methane concentration of 0.5% and bottom-up flow design.

Main Results:

  • Achieved a mean methane removal efficiency of 89.0 ± 6.7%.
  • The system demonstrated consistent performance and enabled visual monitoring.
  • Open-source design files and assembly instructions were provided under a CERN OHL license.

Conclusions:

  • The developed biofiltration system offers a standardized and reproducible solution for methane mitigation.
  • The open-source approach facilitates customization and accelerates the development of scalable biofiltration technologies.
  • This hardware supports advancements in methane control and gas-phase bioprocessing research.