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Updated: Mar 20, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
Published on: January 16, 2016
Structure-guided engineering of membrane-binding regions for surfactant-free solubilization of direct electron
Konatsu Ichikawa1, Taiki Adachi1, Tomoko Miyata2,3
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan. sowa.keisei.2u@kyoto-u.ac.jp.
Abstract:
Membrane-bound alcohol dehydrogenase (ADH) from Gluconobacter oxydans is a direct electron transfer-type biocatalyst for ethanol oxidation. To improve its bioelectrocatalysis, membrane-binding regions of ADH were predicted, resulting in the construction of a soluble ADH variant by enzyme engineering. The variant was purified and characterized using structural and bioelectrochemical approaches.
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