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BrCCX1 Negatively Regulates Calcium-Mediated Clubroot Resistance in Chinese Cabbage.
Jing Zhang1, Xinlei Wang1, Sheng Chen2
1Liaoning Key Laboratory of Genetics and Breeding for Cruciferous Vegetable Crops, College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, China.
Plant, Cell & Environment
|March 19, 2026
Summary
Chinese cabbage clubroot resistance is negatively regulated by BrCCX1, a gene influenced by calcium signals. BrMYB106 positively controls resistance by modulating BrCCX1, offering a pathway for developing resistant cultivars.
Area of Science:
- Plant Pathology
- Molecular Biology
- Genetics
Background:
- Clubroot disease, caused by Plasmodiophora brassicae, severely impacts Chinese cabbage (Brassica rapa L. ssp. pekinensis).
- Understanding the genetic regulation of clubroot resistance is crucial for crop improvement.
Purpose of the Study:
- To investigate the role of Cation/Ca²⁺ exchanger 1 (BrCCX1) in Chinese cabbage clubroot resistance.
- To identify regulatory factors involved in BrCCX1-mediated clubroot resistance.
Main Methods:
- Gene expression analysis in response to Plasmodiophora brassicae infection.
- Functional validation of BrCCX1 in Arabidopsis thaliana and Chinese cabbage using mutant and gene silencing/overexpression approaches.
- Yeast one-hybrid, Dual-luciferase, and GUS activity assays to identify promoter interactions.
- Virus-induced gene silencing (VIGS) and transient overexpression for gene function analysis.
Main Results:
- BrCCX1 expression was induced by P. brassicae and acts as a negative regulator of clubroot resistance.
- Exogenous calcium enhanced clubroot resistance, an effect amplified by BrCCX1 silencing.
- BrMYB106 directly binds to the BrCCX1 promoter and acts as a positive regulator of clubroot resistance.
- BrCCX1's negative regulation of resistance is linked to calcium signaling pathways modulated by BrMYB106.
Conclusions:
- BrCCX1 is identified as a negative regulator in calcium-mediated clubroot resistance in Chinese cabbage.
- BrMYB106 modulates BrCCX1, thereby influencing clubroot resistance.
- The findings provide a basis for developing clubroot-resistant Chinese cabbage cultivars by targeting the BrCCX1 gene.
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