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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

CRISPR

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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Single-Cell CRISPR: An Efficient Strategy for Decoding Plant Cis-Regulatory Complexity.

Yuanhang Zhang1, Xuanxuan Luo1, Hongbin Li2

  • 1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.

Plant Biotechnology Journal
|March 19, 2026
PubMed
Summary
This summary is machine-generated.

Single-cell CRISPR screening identifies crucial cis-regulatory elements controlling gene expression. This technology offers precise insights into complex traits and gene regulation for plant research.

Keywords:
cis‐regulatory elementgenetic screenphenotype selectionregulatory networksingle‐cell CRISPR

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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Area of Science:

  • Genomics
  • Molecular Biology
  • Plant Science

Background:

  • Complex traits arise from intricate gene networks.
  • Understanding cis-regulatory elements (CREs) is key to gene expression control.
  • Single-cell transcriptomics offers cell-type-specific gene expression data.

Purpose of the Study:

  • To review advances in functional genome analysis using single-cell CRISPR (scCRISPR).
  • To highlight scCRISPR's potential for single-cell genetic screening of CREs.
  • To explore scCRISPR applications in plant research.

Main Methods:

  • Integration of single-cell transcriptomics with genome-wide genetic screening.
  • Utilizing the single-cell CRISPR (scCRISPR) system for functional screening.
  • Analysis of cell-type-specific gene expression and regulatory element function.

Main Results:

  • scCRISPR enables precise identification of critical regulatory elements.
  • Novel insights into gene-expression control mechanisms are gained.
  • Advances in diverse strategies for functional genome analysis are summarized.

Conclusions:

  • scCRISPR technology is revolutionizing single-cell genetic screening of CREs.
  • This approach provides a powerful tool for understanding complex traits.
  • Opportunities and challenges exist for applying scCRISPR in plant research.