Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rhodopsin and blindness.

V G Wong, W R Green, P R McMaster

    Transactions of the American Ophthalmological Society
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Systemic immunization with rhodopsin caused blindness in primates. This immune response targeted retinal outer segments, leading to irreversible vision loss and inflammation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Retinal hemorrhages in newborn piglets following cardiopulmonary resuscitation.

    American journal of diseases of children (1960)·1992
    Same author

    Toxicity of intravitreous ceftazidime in primate retina.

    Archives of ophthalmology (Chicago, Ill. : 1960)·1992
    Same author

    Inadvertent corneal button inversion during penetrating keratoplasty.

    Cornea·1992
    Same author

    Generation of anti-AKR/gross murine leukemia virus cytotoxic T lymphocytes (CTL). An analysis of precursor CTL frequencies in the AKR.H-2b and C57BL/6 mouse strains.

    Journal of immunology (Baltimore, Md. : 1950)·1992
    Same author

    Elevated intraocular pressure, pigment dispersion and dark hypopyon in endogenous endophthalmitis from Listeria monocytogenes.

    Survey of ophthalmology·1992
    Same author

    Multicentric gastrointestinal and extraintestinal leiomyosarcomatosis: a case report.

    Journal of the National Medical Association·1992
    Same journal

    The Utility of Color Duplex Ultrasonography in the Diagnosis of Giant Cell Arteritis: A Prospective, Masked Study. (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2018
    Same journal

    Autoimmune Retinopathy: Current Concepts and Practices (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2018
    Same journal

    Erratum: Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2018
    Same journal

    The Relationship Between Ocular Itch, Ocular Pain, and Dry Eye Symptoms (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2018
    Same journal

    The Effects of Phacoemulsification and Intraocular Lens Implantation on Anatomical and Functional Parameters in Patients with Primary Angle Closure: A Prospective Study. (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2017
    Same journal

    Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

    Transactions of the American Ophthalmological Society·2017
    See all related articles

    Area of Science:

    • Ophthalmology
    • Immunology
    • Neuroscience

    Background:

    • Rhodopsin, a key protein in the retinal outer segments, is crucial for vision.
    • The immunopathologic potential of rhodopsin has not been fully elucidated.

    Purpose of the Study:

    • To investigate the effects of systemic immunization with purified homologous rhodopsin on primate vision and retinal structure.
    • To determine if rhodopsin is organ-specific and immunopathologically relevant in inducing retinal disease.

    Main Methods:

    • Systemic immunization of Macaca mulatta primates with purified homologous rhodopsin.
    • Clinical observation for signs of visual impairment and retinal changes.
    • Electroretinography (ERG) to assess retinal function.
    • Histopathological analysis of retinal tissues.

    Related Experiment Videos

  • Immunological assays to detect antibodies and delayed hypersensitivity to rhodopsin.

    • Main Results:

    • Immunization induced progressive blindness characterized by inflammation and retinal degeneration.
    • Pathological findings included perivasculitis, subretinal exudations, and bullous retinal detachments.
    • Electroretinographic responses were abolished in affected animals.
    • Antibodies and delayed hypersensitivity to rhodopsin were detected exclusively in diseased primates.
    • Histology confirmed destructive alterations in the outer retinal segments with a granulomatous inflammatory response.

    Conclusions:

    • Purified homologous rhodopsin is capable of inducing an autoimmune retinal disease in primates.
    • Rhodopsin appears to be organ-specific and immunopathologically relevant in mediating visual system damage.
    • This study highlights the potential of rhodopsin as a target in autoimmune-mediated blindness.