Related Experiment Video
Updated: Mar 21, 2026
06:04
Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain
Published on: May 23, 2019
6.1K
A highly sensitive ratiometric fluorescence pH probe for extracellular pH in mouse brain slice
Liyi Ma1, Yuxuan Lu2, Guangwei She3
1State Key Laboratory of Cryogenic Science and Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Summary
A new high-sensitive ratiometric fluorescence pH probe, SiRho-F-pH, enables early disease detection. This probe accurately measures extracellular pH with unprecedented sensitivity and spatial resolution.
Area of Science:
- Biomedical Engineering
- Chemical Biology
- Analytical Chemistry
Background:
- Accurate extracellular pH detection is crucial for early disease diagnosis and understanding pathogenesis.
- Existing fluorescence pH probes lack the sensitivity required for precise extracellular pH measurements.
Purpose of the Study:
- To develop a high-sensitive ratiometric fluorescence pH probe for accurate extracellular pH detection.
- To characterize the probe's performance in terms of sensitivity, selectivity, reversibility, and biocompatibility.
Main Methods:
- Fabrication of the SiRho-F-pH probe via click reaction between Si-rhodamine and fluorescein.
- Spectroscopic analysis to determine the probe's ratiometric response to pH changes (450 nm excitation).
- Microscopy imaging (fixed cells and mouse brain slices) to evaluate detection capabilities.
Main Results:
- The SiRho-F-pH probe exhibited a 1065-fold change in fluorescence intensity ratio (R680/520) across a pH range of 4.57-9.33.
- In fixed cells, the red/green fluorescence ratio (R/G) showed a 4858-fold change within pH 5.00-8.00.
- The probe successfully visualized subtle extracellular pH variations (pH 6.61-6.71) in mouse brain slices with micrometer resolution.
Conclusions:
- The SiRho-F-pH probe offers high sensitivity and selectivity for extracellular pH detection.
- Its ratiometric fluorescence mode provides reliable measurements with excellent spatial resolution.
- This probe holds significant potential for early disease diagnosis and biological research.
