CRISPR/Cas and isothermal amplification in Pathogen Detection: Applications and future perspectives.

Area of Science:

• Molecular Biology
• Biotechnology
• Diagnostics

Background:

• Conventional pathogen detection methods face limitations in speed and accessibility.
• CRISPR-Cas systems and isothermal amplification (IA) offer potential for rapid, field-deployable diagnostics.
• Integration of these technologies addresses limitations of traditional methods.

Purpose of the Study:

• To systematically review principles, optimization, and advances in CRISPR-Cas and IA-integrated diagnostic platforms.
• To highlight synergistic mechanisms for enhanced detection sensitivity.
• To examine innovative integration strategies and readout modalities.

Main Methods:

• Systematic review of CRISPR-Cas and isothermal amplification integration strategies.
• Analysis of nanomaterial roles in signal amplification and system integration.
• Critical evaluation of various detection readout modalities (fluorescence, LFA, electrochemical, digital).

Main Results:

• Synergistic mechanisms significantly enhance detection sensitivity.
• Innovative integration strategies address compatibility challenges.
• Nanomaterials play a crucial role in signal amplification and integration.
• Various readout modalities show suitability for different applications.

Conclusions:

• CRISPR-Cas and IA integration presents a versatile platform for point-of-care diagnostics.
• Future advances in intelligent design and portable devices will enhance public health and food safety applications.
• Further research is needed to address stability, cost, and standardization challenges.