Related Experiment Video
Updated: Mar 21, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
Recent advances in balancing the growth-production trade-off in Bacillus subtilis: A systematic resource reallocation
Yujue Wang1, Qiang Wang1, Jianghua Chen1
1Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Abstract:
As an excellent industrial chassis, Bacillus subtilis plays a pivotal role in promoting the efficient and sustainable manufacturing of high-value biological products. However, the intrinsic resource trade-off between growth and production remains a formidable barrier for biosynthetic efficiency. Therefore, from the perspective of cellular resource allocation, this review provides a comprehensive analysis through a holistic framework encompassing synthetic toolkits, mechanisms, engineering strategies, and industrial applications. We analyze the fundamental limitations of €resource partitioning, focusing on the proteome allocation competition driven by heterologous synthesis and the global physiological feedback responses triggered by metabolic burden. To address resource constraints, the paper discusses fundamental engineering modules to enhance strain robustness and metabolic capacity, as well as system-level strategies for rational resource allocation. Finally, by showcasing diverse industrial applications, this work provides profound insights into overcoming metabolic trade-offs and lays the foundation for the rational design of high-performance B. subtilis cell factories.
Related Concept Videos
Bioreactor Controls-III
Designing Growth Media for Bioreactors
Production of Antibiotics
Bacterial Growth Curve
Production of Biopesticides
Exponential Growth

