Changes in the proteomic profile of athletes' plasma associated with exercise intensity

High physical activity often results in complex and ambiguous proteomic changes. This study aimed to analyze possible changes in the composition and content of proteins in plasma samples of elite athletes from sports with various activity levels and intensity. We performed proteomic profiling of blood plasma from 93 elite athletes with over eight years of continuous professional experience. High-resolution tandem mass spectra obtained in the data-independent acquisition mode were analyzed using the PLGS algorithm against a library of known protein sequences, and the PowerNovo algorithm for de novo protein sequencing. Combining the two protein identification strategies improved the completeness of the analysis and expanded the number of identified proteins. We identified changes in levels of circulating proteins that distinguish the high-intensity group from other groups by proteins involved in immune response, lipid transport and metabolism, and oxygen and iron transport. Changes in protein levels in biological samples of professional athletes may be associated with the training load intensity and type of sport.