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Genetic and Molecular Epidemiological Characterization of ESBL-Producing Escherichia coli Isolated from University
Mitsuru Matsumura1, Seika Matsumura1, Yuma Ito1
1Department of Clinical Laboratory Science, Faculty of Medical Technology, Teikyo University, Tokyo, Japan.
Background:
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are major contributors to global antimicrobial resistance. Colonization in healthy individuals represents a silent reservoir that links community and healthcare settings. Determining the prevalence and molecular characteristics of ESBL-producing strains in community populations is essential for effective infection control.
Methods:
We enrolled 96 healthy third-year students and collected perianal swabs to detect ESBL-producing E. coli. Antimicrobial susceptibility testing, ESBL genotyping, phylogrouping, and POT-based molecular epidemiological typing were performed to characterize the isolates and identify ST131 and its H30 subclone.
Results:
ESBL-producing E. coli were detected in 7.3% of students (7/96; 95% CI: 3.0-14.4%). All isolates harbored CTX-M-type ESBL genes, and the high-risk clone ST131/H30 was frequently identified. POT-based molecular typing revealed distinct patterns for all isolates, indicating independent acquisition rather than clonal transmission within the cohort.
Conclusion:
The detection of multidrug-resistant ST131-H30 in healthy young adults highlights the potential role of community carriers in introducing high-risk clones into healthcare settings. Strengthened surveillance and integrated One Health-based approaches are needed to curb the spread of antimicrobial resistance.
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