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Updated: Mar 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
SNP panel for the evaluation of genetic diversity and relatedness in red deer (Cervus elaphus)
S Kamiński1, K Oleński1, D Zalewski2
1University of Warmia and Mazury, Department of Animal Genetics, Oczapowskiego 5, 10-718 Olsztyn, Poland.
Abstract:
Blood samples from 385 red deer (Cervus elaphus) acquired during officially approved hunting in different hunting divisions throughout Poland were used to isolate the genomic DNA. All individuals were genotyped by Bovine BeadChip (Illumina) for 54,174 Single Nucleotide Polymorphism (SNP) markers. SNPs of inappropriate clusters, with a marker call rate lower than 95% and with a Minor Allele Frequency (MAF) lower than 0.01, located on sex chromosomes and mitochondrial DNA, were removed. In total, 12,146 SNP markers were included for further analysis. Observed and expected heterozygosity amounted to 0.025 and 0.035, respectively. Among 12,146 markers, a panel of 142 SNPs were selected for relatedness analysis. The selected SNPs were unlinked and had a MAF higher than 0.2. This set of SNPs showed a probability of parentage exclusion of 1.42x10-6 and 9.91x10-19 for one and two known parents, respectively. The probability of identity was estimated at 6.84x10-53. The probabilities obtained in this study are sufficient for the monitoring and effective management of the genetic diversity of red deer in Poland and are a cost-effective complementary tool for forensic applications.
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