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Related Experiment Video

Updated: Mar 21, 2026

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
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Storage of Corneas With Predissected Descemet Membrane in an Active Storage Machine.

Paul Goin1,2, Sylvain Poinard1,3, Oliver Dorado1,3

  • 1Laboratory Biology, Engineering and Imaging for Ophthalmology, BiiO, Faculty of Medicine, Health and Innovation Campus, Jean Monnet University, Saint-Etienne, France.

Translational Vision Science & Technology
|March 20, 2026
PubMed
Summary

The active storage machine (ASM) effectively preserves pre-dissected Descemet membrane endothelial keratoplasty (DMEK) grafts for at least 10 days. This innovation enhances logistical flexibility and surgical planning for endothelial keratoplasty procedures.

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Area of Science:

  • Ophthalmology and Vision Science
  • Tissue Engineering and Regenerative Medicine
  • Biomaterials and Medical Devices

Background:

  • Pre-dissection of corneas in eye banks is standard practice to reduce surgical time for endothelial keratoplasty.
  • Conventional passive storage methods limit the storage duration and flexibility for endothelial grafts.
  • Active Storage Machine (ASM) has previously shown superiority for whole cornea preservation.

Purpose of the Study:

  • To evaluate the feasibility of storing pre-dissected Descemet membrane endothelial keratoplasty (DMEK) grafts using an Active Storage Machine (ASM).
  • To extend the storage viability of endothelial grafts beyond current limitations.
  • To enhance flexibility in surgical planning and graft preparation logistics.

Main Methods:

  • Human corneas were pre-dissected, leaving the Descemet membrane attached centrally.
  • Corneas were stored in either ASM (21 mmHg, 2.6 µL/min) or sealed flasks for 3 and 10 days.
  • Endothelial cell density (ECD), corneal thickness, and expression of key endothelial markers (CD166, NCAM, ZO-1, Na+/K+ATPase, COX-IV) were assessed.

Main Results:

  • Endothelial cell viability remained comparable between ASM and conventional storage groups at both 3 and 10 days.
  • Immunofluorescence analysis showed similar expression of assessed markers (CD166, NCAM, ZO-1, Na+/K+ATPase, COX-IV) in both groups.
  • Corneal thickness was significantly lower in the ASM storage group.

Conclusions:

  • The ASM is suitable for storing pre-dissected DMEK grafts for at least 10 days.
  • This method allows for organized graft preparation runs and safe subsequent storage.
  • Utilizing ASM for DMEK storage simplifies logistics and offers greater flexibility for surgeons.