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Unveiling the Probiotic Properties of Lacticaseibacillus paracasei UFTM 2.9 Through Probiogenomic Analysis
Bárbara R Fonseca1, Yasmin N V Sabino1, Beatriz M O Rocha2
1Federal University of Juiz de Fora, Minas Gerais, Juiz de Fora, Minas Gerais, Brazil.
Molecular Nutrition & Food Research
|March 20, 2026
Summary
Lacticaseibacillus paracasei UFTM 2.9 shows strong probiotic potential, confirmed by genomic analysis and in vitro tests. This lactic acid bacterium (LAB) exhibits metabolic versatility and inhibits harmful bacteria, suggesting its suitability for gut health applications.
Area of Science:
- Microbiology
- Genomics
- Probiotics
Background:
- Lactic acid bacteria (LAB) are Gram-positive bacteria with significant biotechnological applications.
- Lacticaseibacillus spp. are recognized for their potential as probiotics, offering health benefits to hosts.
- Lc. paracasei UFTM 2.9 previously demonstrated in vitro probiotic properties.
Purpose of the Study:
- To characterize the probiotic potential of Lc. paracasei UFTM 2.9 using probiogenomic and in vitro analyses.
- To identify genes associated with probiotic traits and assess safety markers.
- To evaluate the antimicrobial activity of Lc. paracasei UFTM 2.9 against various indicator bacteria.
Main Methods:
- Whole-genome sequencing and bioinformatic analysis to identify genes related to probiotic functions.
- In vitro assays to assess metabolic versatility and antimicrobial activity.
- Detection and confirmation of bacteriocin gene clusters.
Main Results:
- The draft genome of Lc. paracasei UFTM 2.9 contains 170 genes linked to probiotic traits, including stress resistance and vitamin biosynthesis.
- No virulence genes or CRISPR elements were found; two phages were identified.
- Bacteriocin production was confirmed, and Lc. paracasei UFTM 2.9 inhibited all 12 tested indicator bacteria, including pathogenic strains.
Conclusions:
- Lc. paracasei UFTM 2.9 possesses significant genetic potential for probiotic traits, including stress resistance and antimicrobial activity.
- The absence of virulence factors and demonstrated antimicrobial efficacy support its safety and utility.
- Results indicate Lc. paracasei UFTM 2.9 is a promising candidate for probiotic applications, capable of surviving the gastrointestinal tract.
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