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Updated: Mar 22, 2026
Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
Published on: September 3, 2021
Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography
Anna Jaeschke1, Matt S Hepburn2, Alireza Mowla3
1Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Shatin, NT, Hong Kong SAR, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Ma Liu Shui, NT, Hong Kong SAR, China.
Abstract:
Here, we present a protocol combining quantitative micro-elastography (QME) with transmitted light (TL) and immunofluorescence (IF) microscopy to study ovarian elasticity and composition in situ. We describe steps for excising murine ovaries, acquiring QME scans, and fixing the tissue. Subsequently, the tissue is sectioned and imaged with TL microscopy to locate functional components, such as corpora lutea and follicles, and segment them within the QME data. Finally, IF is used to analyze cell and molecular composition. For complete details on the use and execution of this protocol, please refer to Jaeschke et al.1.
