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Published on: February 1, 2022
Bioinspired Hydrogel-Functionalized Microchannel-Gated Junction Field-Effect Transistor for Highly Sensitive
Yulan Zeng1, Sijia Yu1, Huabin Cai1
1Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety and Health, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
This study presents a novel biosensor for detecting glutathione (GSH) using a hydrogel-modified junction field-effect transistor (JFET). The sensor offers high sensitivity and selectivity for GSH detection in biological samples.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Microchannel-based sensing mimics biological ion channels for ionic transport control.
- Field-effect transistor (FET) biosensors offer high sensitivity to charge variations.
- Junction field-effect transistors (JFETs) provide a sensitive detection platform.
Purpose of the Study:
- To develop a sensitive JFET-based biosensor for glutathione (GSH) detection.
- To utilize a Schiff-base crosslinked hydrogel within a microchannel as a tunable resistor.
- To leverage changes in hydrogel properties upon GSH interaction for signal transduction.
Main Methods:
- Fabrication of a microchannel device incorporating a hydrogel resistor.
- Formation of the hydrogel via Schiff-base crosslinking of aldehyde-modified hyaluronic acid (AHA) and DTP.
- Detection of GSH by monitoring changes in JFET channel current due to hydrogel network disruption.
Main Results:
- The biosensor demonstrated a linear response to GSH from 100 nM to 1.00 mM.
- A low detection limit of 38.1 nM for GSH was achieved.
- The sensor showed excellent sensitivity, selectivity, and reproducibility in diluted human serum.
Conclusions:
- The developed JFET biosensor offers a novel and highly sensitive method for GSH detection.
- The hydrogel-based tunable resistor strategy provides a versatile platform for biosensing.
- This approach holds potential for clinical diagnostics and disease monitoring.
