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Related Concept Videos

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Co-Immobilized Multi-Enzyme Systems with Spatial Arrangement for Glucose Detection.

Huihui Li1, Meijia Song1, Shumao Yang1

  • 1State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education; Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, China.

Journal of Agricultural and Food Chemistry
|March 20, 2026
PubMed
Summary

A novel compartmentalized system precisely arranges enzymes for enhanced glucose detection. This method improves sensitivity and stability, offering a promising tool for practical glucose monitoring.

Keywords:
carbon spherescompartmentalized co-immobilized multi-enzymatic systemglucose detectionpolydopamine

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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Area of Science:

  • Biotechnology
  • Biosensors
  • Materials Science

Background:

  • Traditional random co-immobilization of enzymes for glucose detection lacks spatial control, hindering catalytic efficiency and sensitivity.
  • Hydrogen peroxide (H₂O₂) inhibition is a significant challenge in multi-enzyme systems for glucose sensing.
  • Hierarchical porous carbon spheres offer a versatile platform for enzyme immobilization.

Purpose of the Study:

  • To develop a compartmentalized multi-enzyme system for enhanced glucose detection with precise spatial control.
  • To investigate the impact of localized enzyme arrangement on catalytic efficiency and H₂O₂ inhibition.
  • To evaluate the stability, reusability, and sensitivity of the developed glucose biosensor.

Main Methods:

  • Fabrication of hierarchical porous carbon spheres functionalized with polydopamine (PDA).
  • Compartmentalized co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) within PDA layers on carbon spheres.
  • Characterization of enzyme loading, spatial arrangement, and catalytic performance of the HRP.PDA.GOx@NPC-10 system.

Main Results:

  • Achieved high immobilization yields for GOx (54.6%) and HRP (65.0%) with controlled spatial localization.
  • The HRP.PDA.GOx@NPC-10 system demonstrated 1.65-fold higher activity compared to free enzymes.
  • Exhibited excellent stability under extreme pH and temperature, retaining 75.66% activity after 7 cycles.
  • Achieved a low glucose detection limit of 0.28 μM with a rapid reaction time of 3 minutes.

Conclusions:

  • The compartmentalized enzyme immobilization strategy effectively enhances glucose detection sensitivity and stability.
  • The HRP.PDA.GOx@NPC-10 system shows significant potential for practical and sensitive glucose monitoring applications.
  • Precise spatial control of enzyme arrangement is crucial for overcoming limitations in multi-enzyme biosensors.