Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteasome Dysfunction and Aggregation-Prone Proteins in Neurodegenerative Diseases: From Mechanisms to Therapeutic Opportunities.

International journal of molecular sciences·2026
Same author

Cardiorespiratory fitness, genetic susceptibility, and the risk of chronic obstructive pulmonary disease: findings from observational and two-sample bidirectional Mendelian randomisation analyses.

BMC medicine·2026
Same author

Non-invasive continuous versus intermittent oscillometric arterial pressure monitoring and maternal hypotension during cesarean delivery: a randomized controlled trial.

Scientific reports·2026
Same author

Development of a customizable vascularization strategy using a modular microfluidic platform for fibroblast-free vascularized tissue.

Materials today. Bio·2026
Same author

Pathogenic roles of the IMNEPD-associated PTRH2 mutants in aggravating mitochondrial dynamics and its rescue.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology.

Molecular neurodegeneration·2026

Related Experiment Video

Updated: Mar 22, 2026

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
10:38

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

Published on: December 16, 2022

2.9K

Phenotypic profiles for anticancer drug responses using tumor spheroids.

Youngwon Kim1, Jiseok Lim2, Bongseop Kwak3

  • 1College of Medicine, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyangsi, Gyeonggi-do 10326, Republic of Korea.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 20, 2026
PubMed
Summary

This study introduces a new method using tumor spheroids to test breast cancer drugs. It offers standardized criteria for evaluating drug effectiveness based on observable changes.

Keywords:
Breast cancer drug exposureDrug screeningMorphological and optical analysisPhenotypic drug discoveryScreening guideline developmentTumor spheroid

More Related Videos

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
10:03

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

9.4K
Generation of 3D Tumor Spheroids for Drug Evaluation Studies
10:33

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Published on: February 24, 2023

3.1K

Related Experiment Videos

Last Updated: Mar 22, 2026

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
10:38

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

Published on: December 16, 2022

2.9K
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
10:03

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

9.4K
Generation of 3D Tumor Spheroids for Drug Evaluation Studies
10:33

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Published on: February 24, 2023

3.1K

Area of Science:

  • Oncology
  • Drug Discovery
  • Biotechnology

Background:

  • Phenotypic drug discovery evaluates anticancer drug responses without target knowledge.
  • Tumor spheroids mimic in vivo tumor architecture better than 2D cultures.
  • Standardized drug evaluation criteria for spheroids are lacking.

Purpose of the Study:

  • To develop a standardized, phenotype-based evaluation system for breast cancer chemotherapy using tumor spheroids.
  • To establish quantifiable parameters for drug screening and evaluation.
  • To enhance the utility of spheroids in phenotypic anticancer drug discovery.

Main Methods:

  • Fabricated uniform tumor spheroids from triple-positive (ER+, PR+, HER2+) invasive ductal carcinoma cells.
  • Administered breast cancer anticancer drugs at clinically relevant plasma concentrations.
  • Proposed and monitored drug response evaluation parameters: spheroid size change, morphological features (surface cell size, convexity), and optical transmittance.

Main Results:

  • Developed optical image-based analytical features for drug response evaluation.
  • Demonstrated consistent correlations between analytical features, molecular mechanisms of action, and drug response.
  • Validated the proposed parameters during drug treatment and post-exposure observation.

Conclusions:

  • Established a phenotype-based evaluation system for breast cancer chemotherapy using tumor spheroids.
  • Proposed quantifiable parameters for drug screening and evaluation.
  • Provided a reference model and data for assessing investigational compounds in phenotypic drug discovery.