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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Double-clamp Schiff base ligand engineered Ru (II) complex exhibiting Zn2+-activated fluorescence for selective Zn2+
Longlong Li1, Qiang Zhang1, Gangyi Sun1
1College of Materials Science and Engineering, Weifang University of Science and Technology, Shandong, 262700, China.
Abstract:
Zinc ions (Zn2+) are essential trace elements for the human body, yet their dysregulation can lead to numerous diseases. Consequently, Zn2+ sensing and imaging in living cells have attracted substantial scientific interest. In this work, a novel visible light-excited fluorescence sensor, [Ru (FPPM) (bipy)2]2+ (λex = 445 nm), was developed by incorporating double-clamp bridging ligand (FPPM) into Ruthenium (II) complex, thereby coupling the photoinduced electron transfer (PET) mechanism with CN isomerization. This complex functions as an enhanced fluorescence sensor (λem = 623 nm) for the selective and sensitive detection of Zn2+ in PBS (pH = 7.2-7.4). The coordination stoichiometry between [Ru (FPPM) (bipy)2]2+ and Zn2+ was determined to be 1:1, with a high stability constant (K = 5.2792 × 104). The limit of detection (LOD) and limit of quantification (LOQ) were calculated as 5.2855 × 10-8 mol/L and 1.7618 × 10-7 mol/L, respectively. Time-dependent density functional theory (TDDFT) computations were employed to elucidate the mechanism by which Zn2+ enhances the fluorescence of [Ru (FPPM) (bipy)2]2+. Furthermore, the [Ru (FPPM) (bipy)2]2+ with low cytotoxicity enables Zn2+ detection in living cells through fluorescence imaging.
