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A Label-Free Optical Biosensor Based on Ordered Porous Layer Interferometry for IgG Quantification in Milk.

Yuxin Zhai1, Lu Wang1,2, Dongmin Zhao3

  • 1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096 China.

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Summary

A new label-free optical biosensor accurately quantifies Immunoglobulin G (IgG) in milk in real-time. This technology enables rapid quality control throughout dairy production.

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Area of Science:

  • Biosensing technologies
  • Dairy science
  • Analytical chemistry

Background:

  • Immunoglobulin G (IgG) levels are key indicators of milk quality.
  • Conventional methods for IgG measurement are complex and time-consuming.
  • There is a need for rapid, real-time monitoring of IgG in dairy production.

Purpose of the Study:

  • To develop a label-free optical biosensor for rapid IgG quantification in milk.
  • To utilize ordered porous layer interferometry (OPLI) for sensitive IgG detection.
  • To enable real-time monitoring of IgG across various dairy processing stages.

Main Methods:

  • Direct sample application to a flow cell for analysis.
  • Immobilization of Protein A ligand onto the sensor surface via chemical conjugation.
  • Optimization of ligand immobilization, flow rate, contact time, and regeneration to minimize non-specific binding and enhance accuracy.

Main Results:

  • The OPLI biosensor achieved a linear quantification range of 0.44 μg/mL to 5 mg/mL.
  • The limit of quantification was determined to be 0.44 μg/mL, suitable for raw to finished milk.
  • High reproducibility was observed, with low instrumental and batch-to-batch relative standard deviations.

Conclusions:

  • The OPLI biosensor provides a sensitive, real-time, and wide-range method for IgG analysis.
  • This technology facilitates effective quality control in dairy production.
  • The developed biosensor is applicable throughout the dairy production chain for IgG assessment.