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Related Concept Videos

Imaging Studies for Cardiovascular System V: CT01:28

Imaging Studies for Cardiovascular System V: CT

Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT01:25

Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT

Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Imaging Studies VII: Vascular Imaging01:19

Imaging Studies VII: Vascular Imaging

DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...

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Related Experiment Video

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High Throughput Single-cell and Multiple-cell Micro-encapsulation
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A 3D-Printed Compact Multi-Nozzle Microfluidic Device for Scalable Microencapsulation of Pluripotent Stem Cells.

Quoc Huynh Nguyen1, Kianna Nguyen1, Quang Tuan Che1

  • 1Department of Physiological and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA.

Advanced Healthcare Materials
|March 21, 2026
PubMed
Summary

This study introduces a 3D printed microfluidic device for high-throughput encapsulation of human pluripotent stem cells (hPSCs). The novel system efficiently generates microcapsules, preserving cell pluripotency for biomedical applications.

Keywords:
3D printedcell encapsulationcore–shell microcapsulemicrofluidicstem cell

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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Microfluidics

Background:

  • Encapsulation of human pluripotent stem cells (hPSCs) is crucial for tissue engineering, drug screening, and cellular therapies.
  • Existing methods often face a trade-off between capsule complexity and production speed.
  • Microcapsules facilitate hPSC organization, in vitro differentiation, and in vivo immunoisolation.

Purpose of the Study:

  • To develop a novel microfluidic device for efficient and structurally complex microcapsule fabrication.
  • To overcome the limitations of current encapsulation techniques regarding throughput and complexity.
  • To enable scalable encapsulation of hPSCs for therapeutic applications.

Main Methods:

  • A novel 3D printed microfluidic device was designed and fabricated.
  • The device utilizes multiple nozzles for high-throughput microcapsule generation.
  • Human pluripotent stem cells (hPSCs), including hESCs and iPSCs, were encapsulated within hydrogel microcapsules.

Main Results:

  • The 3D printed device achieved high-throughput fabrication of microcapsules (up to 1825 Hz).
  • A 10-nozzle device demonstrated a tenfold increase in production rate compared to a single-nozzle device.
  • Encapsulated hPSCs formed spheroids/embryoid bodies and maintained pluripotency.

Conclusions:

  • The developed 3D printed microfluidic technology enables efficient, high-throughput encapsulation of hPSCs.
  • This method produces structurally complex microcapsules suitable for various biomedical applications.
  • The technology supports the large-scale encapsulation of hPSCs necessary for clinical treatments.