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Updated: Mar 23, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Discovery of pyrimidine-based small activators of insulin degrading enzyme
Jamal El Bakali1, Cyril Ronco1, Valérie Landry1
1Univ. Lille, Inserm, Institut Pasteur de Lille, U1177 - Drugs and Molecules for Living Systems, Lille, F-59000, France.
Abstract:
Insulin-Degrading Enzyme IDE, a zinc metalloprotease that has been associated with Alzheimer 's disease, metabolic diseases and cancer, is an attractive target. Its complex structure, substrate preferences, non-catalytic activities require the development of modulators of this enzyme. While several potent inhibitors are now available, we still lack activators for both in vitro and in vivo explorations of IDE functions and potential application in Alzheimer 's disease. Here we describe the discovery of an original pyrimidine-based series of IDE activators by screening and its structure-activity relationships. Docking studies suggest that compounds may bind the IDE dimer interface, and sterically increase IDE catalytic activity. In vitro and in vivo activities of best activator are consistent with an increase in insulin hydrolysis by IDE in the presence of the activator BDM71230. This compound can serve as a pharmacological tool to explore this intriguing enzyme.
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