Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

19.7K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
19.7K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

19.2K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
19.2K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

830
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
830
Sanger Sequencing01:57

Sanger Sequencing

777.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
777.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Construction and Practice of the "Leading Goose Navigation and Four-Dimensional Synergy" Model for Cultivating Top Innovative Talents in Forensic Medicine].

Fa yi xue za zhi·2026
Same author

Differential thermal stability of mRNA, miRNA and lncRNA in purified human tissue-derived RNA extracts: a proof-of-principle study with forensic implications.

Legal medicine (Tokyo, Japan)·2026
Same author

A Preliminary Study of Pig Carcass Decomposition and Necrophagous Fly Community Dynamics on Hainan Island, China.

Insects·2026
Same author

Estimating time of day from fingertip blood samples using RNA molecules with diurnal oscillating expression: a proof-of-principle study.

International journal of legal medicine·2026
Same author

ML-Powered MultiFluid-mRNAplex31: A Self-Developed Panel for Precise Tracing of Five Body Fluids.

Genomics, proteomics & bioinformatics·2026
Same author

An in silico evaluation of tri-allelic SNPs from 1kGP Phase IV data for distant kinship identification and mixture analysis.

Forensic science international. Genetics·2026

Related Experiment Video

Updated: Mar 23, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.4K

RNA-STR/SNP: A mirror of forensic DNA-STR/SNP.

Zhiyong Liu1, Jiajun Liu2, Riga Wu2

  • 1Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou 510080, China; Guangzhou Forensic Science Institute, Guangzhou 510442, China.

Forensic Science International. Genetics
|March 21, 2026
PubMed
Summary

Forensic RNA genotyping of short tandem repeat (STR) and single nucleotide polymorphism (SNP) markers from blood and semen is possible, yielding results consistent with DNA profiles. This opens new avenues for analyzing compromised forensic samples.

Keywords:
DNAForensic geneticsRNASNPSTRmtDNA

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.8K
A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

21.5K

Related Experiment Videos

Last Updated: Mar 23, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.4K
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.8K
A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

21.5K

Area of Science:

  • Forensic genetics
  • Molecular biology
  • Genomics

Background:

  • Short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) are crucial for DNA-based individual identification and paternity testing.
  • RNA polymorphisms, specifically mRNA SNPs, are gaining attention for body fluid identification and contributor assignment.
  • The feasibility of genotyping common forensic DNA markers at the RNA level remains largely unexplored.

Purpose of the Study:

  • To investigate whether common forensic DNA short tandem repeat (STR) and single nucleotide polymorphism (SNP) markers can be genotyped from RNA.
  • To assess the concordance of RNA-derived genotypes with genomic DNA (gDNA) profiles.
  • To explore the potential of RNA genotyping for forensic applications, particularly with compromised samples.

Main Methods:

  • Peripheral blood and semen samples were analyzed for RNA genotyping.
  • A massively parallel sequencing (MPS)-based panel was used, comprising 204 STRs, 142 SNPs, and mtDNA hypervariable regions (HVRs).
  • Genomic DNA (gDNA) was analyzed in parallel for validation and comparison.

Main Results:

  • Several common STR loci (e.g., CSF1PO, D18S51) were successfully genotyped from blood and/or semen RNA at high frequencies, with genotypes matching gDNA.
  • Sex chromosomal STR loci were efficiently genotyped in blood RNA, and specific STRs were highly represented in semen RNA, showing concordance with gDNA.
  • Sample-type-specific genotyping was observed for certain loci, and some autosomal SNPs and mtDNA fragments were successfully genotyped from both blood and semen RNA.

Conclusions:

  • RNA can provide partial STR and SNP genotypes that are consistent with gDNA profiles.
  • Evidence suggests body fluid-specific expression patterns for certain RNA markers.
  • RNA genotyping holds significant potential for forensic analysis of compromised samples, marking a new era in forensic RNA analysis.