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CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Mar 23, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

Virus-mediated genome editing in grasses.

Changyi Zhan¹, Jiacheng Hu², Yongliang Zhang¹

¹State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

Trends in Biotechnology

|March 21, 2026

Summary

This summary is machine-generated.

Genetic transformation in grasses is difficult, hindering genome editing. Plant viral vectors provide a new method for heritable editing in grasses without tissue culture, advancing genome-editing platforms.

Keywords:

delivery heritable editing plant virus-based vectors tissue culture-free transgene-free

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Last Updated: Mar 23, 2026

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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Genetic transformation in monocots, particularly grasses, presents significant challenges.
These challenges limit the application of advanced genome-editing technologies in important grass species.

Purpose of the Study:

To explore plant viral vectors as an alternative method for genetic transformation in grasses.
To assess the potential of viral vector systems for enabling heritable genome editing in monocots.

Main Methods:

Utilized representative plant viral vector systems, including barley stripe mosaic virus and barley yellow striate mosaic virus.
Applied these systems to several grass species to evaluate transformation efficiency and editing capabilities.

Main Results:

Demonstrated that plant viral vectors can facilitate genetic transformation in grasses.
Showcased the ability of these systems to achieve heritable genome editing in multiple grass species.
Confirmed the potential of viral vectors as a tissue culture-free alternative.

Conclusions:

Plant viral vectors offer a promising, transformation- and tissue culture-free approach for genetic modification in grasses.
These systems represent a viable platform for developing advanced genome-editing tools in monocots.
Overcoming transformation barriers in grasses can significantly expand genome editing applications.