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Published on: April 26, 2024
Reprogramming regioselectivity of nitrile hydratase towards adiponitrile via semi-rational design of a key "switch"
Yi Guo1, Yingjie Song2, Guobing Chen2
1School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, China; Laboratory of Synthetic Biology and Biotransformation, Chengdu Research Institute of Dalian University of Technology, Chengdu, 611939, China.
Abstract:
5-Cyanovaleramide (5-CVAM) is a monocyanoamide intermediate used for synthesizing herbicides and fine chemicals. Compared to chemical catalysis, nitrile hydratase (NHase, EC 4.2.1.84) is a green catalyst for the conversion of nitrile to amide. In our previous work, we heterologously expressed the NHase gene from Rhodococcus erythropolis CCM2595 (ReNHase) in Escherichia coli (E.coli) by optimizing the ribosomal binding site (RBS), obtaining a Fe-type ReNHase with balanced expression of multiple subunits, structural integrity, and high catalytic activity towards adiponitrile (ADN). However, this was accompanied by a significant decrease in 5-cyanovaleramide regioselectivity, leading to massive production of the by-product adipamide (ADM). This study used a semi-rational enzyme engineering strategy, screening mutations in selected residues within the substrate channel and binding pocket. We identified the key 'switch' site βTyr72 that regulates ReNHase's regioselectivity. Mutants βY72A and βY72C were obtained, with βY72C achieving 100% regioselectivity for 5-CVAM. Kinetic parameters showed a 5.1-fold decrease in the catalytic efficiency kcat/Km of βY72C towards 5-CVAM (from 1.75 mM-1 s-1 to 0.34 mM-1 s-1). Molecular docking and dynamics analysis indicated that the mutation at βY72 weakened hydrogen bonding interactions between NHase and ADN/5-CVAM, and increased enzyme structural flexibility, reducing binding stability. This likely limited the reaction rate of ReNHase catalyzing ADN and inhibited 5-CVAM conversion to ADM, achieving high 5-CVAM selectivity. This study provides a reference for artificially regulating NHase regioselectivity.
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