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Updated: Mar 24, 2026

Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts

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Published on: August 26, 2021

Recellularized Humanized Bioengineered Biatrial Model for Arrhythmia, Biological Pacemakers, and Optogenetic Studies.

Matteo Ghiringhelli¹, Daniel Shiff¹, Harel Grinstein¹

¹Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion-Israel Institute of Technology, Haifa (M.G., D.S., H.G., T.K., A.G., O.E., M.L., G.A., A.S., Y.W., S.G., I.H., L.G.).

Circulation Research

|March 23, 2026

Summary

Researchers created a novel light-controllable human atrial tissue model using stem cells and optogenetics. This engineered heart tissue can model atrial arrhythmias and test new therapies like optogenetic cardioversion.

Keywords:

action potentials atrial fibrillation myocytes, cardiac optogenetics pluripotent stem cells

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Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

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Last Updated: Mar 24, 2026

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Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

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Published on: November 7, 2019

Area of Science:

Cardiovascular Research
Regenerative Medicine
Optogenetics

Background:

Studying atrial arrhythmias is challenging due to a lack of suitable human cardiac tissue models.
Existing models do not allow for targeted, functional perturbations essential for research.

Purpose of the Study:

To engineer anatomically relevant, light-sensitive human atrial chambers for studying arrhythmias.
To develop a model for testing novel therapeutic interventions.

Main Methods:

Combined human pluripotent stem cells, differentiation protocols, and rat heart decellularization/recellularization.
Utilized optogenetics (ChR2/CoChR) for light-controlled pacing and stimulation.
Confirmed atrial identity and architecture through immunostaining, optical mapping, and pharmacology.

Main Results:

Successfully created bioengineered atria with preserved architecture and function.
Demonstrated optogenetic pacing using stem cell-derived sinoatrial nodal cells.
Induced and mapped reentrant arrhythmias, providing insights into atrial fibrillation mechanisms.
Showcased optogenetic cardioversion for terminating induced arrhythmias.

Conclusions:

Established a novel, light-controllable, bioengineered humanized biatrial tissue model.
The model is suitable for studying atrial arrhythmias, drug testing, and disease modeling.
Enables evaluation of innovative therapies like biological pacemaking and optogenetics.