Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virtual reality for oral health education in a community-based after-school outreach program.

Advances in physiology education·2026
Same author

Caregiving Demands and Depression Symptoms among Caregivers of Individuals with Down Syndrome during the COVID-19 Pandemic.

medRxiv : the preprint server for health sciences·2026
Same author

Efficacy of 0.5M mannitol as an adjuvant to lidocaine and epinephrine for intra-oral nerve blocks - a split-mouth, randomized controlled trial.

BMC oral health·2026
Same author

Inpatient pharmacist impact on guideline directed medical therapy in heart failure with reduced ejection fraction.

Journal of the American Pharmacists Association : JAPhA·2026
Same author

Symptomatic Changes Associated With a Pharmacist-Led COPD Program.

Chronic obstructive pulmonary diseases (Miami, Fla.)·2026
Same author

Detrended fluctuation analysis of amygdala-hippocampal beta synchrony reveals network rigidity in depression associated with temporal lobe epilepsy.

Journal of neural engineering·2026

Related Experiment Video

Updated: Mar 24, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

11.8K

Single-Nucleus Transcriptomics Reveals Cell Type-Specific Remodeling and Epilepsy-Associated Microglia.

Victoria Ho1,2, Ruth Tjondropurnomo1, Jennifer Nguyen1,3

  • 1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

Biorxiv : the Preprint Server for Biology
|March 23, 2026
PubMed
Summary

Mesial temporal lobe epilepsy (TLE) involves hippocampal changes. Researchers identified specific cell alterations, including a novel epilepsy-associated microglia (EAM) population, offering insights into seizure development.

More Related Videos

Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.7K
Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease
04:22

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

1.5K

Related Experiment Videos

Last Updated: Mar 24, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

11.8K
Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.7K
Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease
04:22

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

1.5K

Area of Science:

  • Neuroscience
  • Epileptology
  • Genomics

Background:

  • Mesial temporal lobe epilepsy (TLE) is the most common acquired epilepsy, often linked to head trauma.
  • TLE causes difficult-to-treat seizures and cognitive impairments.
  • The cellular and genetic drivers of TLE and its cognitive effects remain unclear.

Purpose of the Study:

  • To investigate gene expression patterns and cell types involved in epileptogenesis in TLE.
  • To identify molecular mechanisms underlying cognitive deficits in TLE.

Main Methods:

  • Single nucleus RNA sequencing was performed on mouse hippocampal tissue.
  • Samples were collected 3 and 6 weeks after pilocarpine-induced status epilepticus, a TLE model.
  • Transcriptomic profiles and cell-cell interactions were analyzed.

Main Results:

  • Reduced Cck and Lamp5-Lhx6 interneuron subclusters were observed.
  • Increased Cajal-Retzius cells, dentate granule (DG) cell precursors, and a mature DG cell subcluster were found.
  • A distinct epilepsy-associated microglia (EAM) subcluster was identified, showing partial overlap with microglia in Alzheimer's disease and traumatic brain injury models, with enriched Myo1e and Igf1 genes.

Conclusions:

  • The study defines transcriptomic programs underlying cellular alterations in TLE.
  • Identified epilepsy-associated microglia (EAM) and their interactions with DG cells provide mechanistic insights.
  • This dataset facilitates further research into the processes driving epileptogenesis and cognitive deficits in TLE.