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Updated: Mar 24, 2026
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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
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Restraint Induces Sickness Responses Independent of Injection with Epstein-Barr Virus (EBV)-Encoded dUTPase.
Taryn G Aubrecht1, Bachir Abi Salloum1, Maria Eugenia Ariza2
1Departments of Neuroscience, The Ohio State University, Columbus, OH, USA.
Journal of Behavioral and Brain Science
|March 23, 2026
Summary
Acute stress did not enhance sickness responses from Epstein-Barr virus (EBV)-encoded dUTPase in mice. This suggests EBV-dUTPase sickness responses differ between acute and chronic stress.
Area of Science:
- Virology
- Neuroimmunology
- Stress Physiology
Background:
- Epstein-Barr virus (EBV) infects most adults and is linked to chronic fatigue syndrome.
- EBV-encoded dUTPase can induce sickness responses, which are exacerbated by chronic stress.
- Individuals often adapt to chronic stress, prompting investigation into acute stress effects.
Purpose of the Study:
- To test if acute restraint stress potentiates sickness responses to EBV-encoded dUTPase.
- To investigate the differential impact of acute versus chronic stress on EBV-dUTPase-induced sickness.
Main Methods:
- Male CD-1 mice received daily injections of saline or EBV-encoded dUTPase for one or three days.
- Mice were subjected to daily three-hour restraint stress or left undisturbed.
- Weight gain and anxiety-like behaviors were assessed.
Main Results:
- Restraint decreased weight gain in both one- and three-day experiments.
- Three days of restraint induced sickness responses, independent of dUTPase.
- Acute restraint stress did not alter sickness responses associated with EBV-encoded dUTPase.
Conclusions:
- Acute restraint stress does not potentiate sickness responses to EBV-encoded dUTPase.
- Sickness responses to EBV-dUTPase appear to be modulated differently by acute versus chronic stress.
- Further research is needed to understand the mechanisms underlying these stress- and EBV-dUTPase interactions.
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