Integrative Multi-Omics and Single-Cell Profiling Identify Chitinase Domain Containing Protein 1 (CHID1) as a...

Area of Science:

• Oncology
• Molecular Biology
• Genomics

Background:

• Glioblastoma multiforme (GBM) is an aggressive brain tumor with high recurrence rates.
• The chitinase and chitinase-like protein family has diverse roles, but CHID1's function in GBM is unknown.
• Chitinase-like proteins are recognized as mesenchymal-associated markers.

Purpose of the Study:

• To investigate the expression patterns and biological significance of CHID1 in GBM.
• To explore the association of CHID1 with transcriptional programs and potential therapeutic targets in GBM.

Main Methods:

• Integrative multi-omics analysis using TCGA-GBM and CGGA transcriptomic datasets.
• Single-cell RNA sequencing and pathway enrichment analyses (GSEA, GO, KEGG, MetaCore).
• Pharmacogenomic correlations and molecular docking for drug-response exploration.

Main Results:

• CHID1 expression is significantly higher in GBM compared to normal brain tissue.
• Elevated CHID1 levels are associated with poorer overall survival in GBM patients.
• Single-cell analysis revealed tumor-associated CHID1 expression patterns.
• Pathway analysis linked CHID1 to oxidative phosphorylation, redox processes, DNA repair, and cell cycle pathways.

Conclusions:

• CHID1 is a GBM-associated transcriptional marker.
• CHID1 expression is linked to critical metabolic and redox-related pathways in GBM.
• This study provides a comprehensive resource for understanding chitinase family roles in GBM.