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Updated: Mar 24, 2026

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
Published on: July 26, 2022
SHED-derived exosomes ameliorate age-related osteoporosis by activating mitophagy in senescent bone marrow
Kuan Yang1,2, Baize Zhang3, Yingyue Zhang1,2,3
1Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, China.
Aim:
This study aims to explore the therapeutic efficacy of stem cells from human exfoliated deciduous teeth-derived exosomes (SHED-Exo) in age-related osteoporosis (OP) and clarify its mechanism via mitophagy activation in senescent bone marrow mesenchymal stem cells (BMSCs).
Methods:
SHED-Exo were isolated, characterized and proteomically profiled. In vitro, H2O2-induced senescent bone marrow mesenchymal stem cells (BMSCs) were treated with SHED-Exo, with mitophagy modulators used for mechanistic validation. In vivo, SHED-Exo were systemically administered to aged osteoporotic mice, assessing biodistribution, safety and therapeutic effects via micro-computed tomography, and molecular analyses of bone tissue/BMSCs.
Results:
SHED-Exo possessed canonical exosomal properties and were enriched in osteogenic/mitophagic proteins. In vitro, SHED-Exo restored mitochondrial function, activated mitophagy and enhanced osteogenic differentiation of senescent BMSCs. In vivo, SHED-Exo showed no organ toxicity and effectively ameliorated bone loss by upregulating mitophagic/osteogenic markers in aged osteoporotic mice.
Conclusion:
SHED-Exo ameliorate age-related OP by activating mitophagy in senescent BMSCs, serving as a novel translational nanotherapeutic for age-related bone disorders.
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