Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proofreading

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore, it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...

Proofreading

Proofreading

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Step-Growth Polymerization: Overview

Step-Growth Polymerization: Overview

Step-growth or condensation polymerization is a stepwise reaction of bi or multifunctional monomers to form long-chain polymers. As all the monomers are reactive, most of the monomers are consumed at the early stages of the reaction to form small chains of reactive oligomers, which then combine to form long polymer chains in the late stages. Hence, the reaction has to proceed for a long time to achieve high molecular weight polymers.
Many natural and synthetic polymers are produced by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A <i>De Novo</i> CO<sub>2</sub> Reductase Featuring a Cysteine-Ligated Cobalt Porphyrin Cofactor.

Journal of the American Chemical Society·2026

Same author

Single-atom substitution redirects KatG reactivity from cofactor biogenesis to stereoselective sulfoxidation.

Nature communications·2026

Same author

Mechanism of Photoinduced Conformational Changes in the Photoenzyme Fatty Acid Photodecarboxylase Revealed by Light- Footprinting Ion Mobility Mass Spectrometry.

Journal of the American Chemical Society·2026

Same author

The Mycobacterium tuberculosis Rv0132c Gene Product Mtb-FGD2 Can Act as an F<sub>420</sub>-Dependent Glucose Dehydrogenase.

Proteins·2026

Same author

Biocatalytic Production of a Key Chiral Intermediate of the HIV Capsid Inhibitor Lenacapavir.

Journal of the American Chemical Society·2026

Same author

Structure and characterisation of CMP-Kdn synthetase from the haptophyte microalgae <i>Prymnesium parvum</i>.

RSC chemical biology·2026

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Directed Evolution of an Efficient Polycarbonate Depolymerase With Exceptional Operational Stability.

Henry A Jones¹, Amy E Hutton^1,2, Dominic Harris-Jukes³

¹Manchester Institute of Biotechnology, School of Chemistry, The University of Manchester, Manchester, UK.

Angewandte Chemie (International Ed. in English)

|March 23, 2026

Summary

Researchers engineered plastic-degrading enzymes to recycle polycarbonate, a valuable polymer. This directed evolution platform rapidly breaks down polycarbonate into its basic components for efficient recycling.

Keywords:

biocatalysis depolymerization directed evolution polycarbonate polymer hydrolase

More Related Videos

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Experiment Videos

Last Updated: Mar 24, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Area of Science:

Biotechnology
Polymer Science
Enzyme Engineering

Background:

A high-throughput directed evolution platform was previously developed for engineering polymer-degrading enzymes.
This platform was successfully used to create HotPETase, an efficient and thermostable variant of IsPETase.

Purpose of the Study:

To demonstrate the utility of the directed evolution platform for re-engineering PET-degrading enzymes to recycle other aromatic-containing commodity polymers.
To engineer a promiscuous poly(bisphenol-A carbonate) (PC) depolymerase activity of LCC^ICCG into an efficient polycarbonate hydrolase.

Main Methods:

Directed evolution was employed to enhance the poly(bisphenol-A carbonate) (PC) depolymerase activity of LCC^ICCG.
The engineered enzyme was evaluated for improved solvent tolerance, operational stability at elevated temperatures, and alleviation of enzyme-concentration dependent inhibition.

Main Results:

An engineered polycarbonate hydrolase, termed PC-2, was developed with enhanced activity and stability.
PC-2 demonstrated improved solvent tolerance and operational stability at elevated temperatures.
Enzyme-concentration dependent inhibition observed in the parent enzyme was alleviated in PC-2.
PC-2 achieved rapid and complete depolymerization of a PC film to bisphenol-A (BPA) within 6 hours at 75°C.

Conclusions:

The directed evolution platform is effective for adapting plastic-degrading enzymes to new polymer classes.
Engineered enzymes can be readily developed for the efficient recycling of valuable commodity polymers like polycarbonate.
This approach holds promise for advancing sustainable plastic recycling technologies.