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Development of an Efficient Murine Tissue Expansion Model for Skin Regeneration Research
Xing Huang1, Peiyi Li2, Rui Jin2
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine; Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine.
Journal of Visualized Experiments : Jove
|March 23, 2026
Summary
This study developed a standardized rat model for tissue expansion to improve skin regeneration. The model mimics human tissue expansion, aiding research into optimizing clinical protocols for wound healing.
Area of Science:
- Regenerative Medicine
- Biomedical Engineering
- Dermatology
Background:
- Skin regeneration is crucial for wound healing and can be enhanced by mechanical stimulation.
- Tissue expansion accelerates skin regeneration but faces limitations due to mechanical loading and regenerative capacity mismatch.
- A standardized animal model is needed to study tissue expansion and optimize clinical applications.
Purpose of the Study:
- To establish a reproducible preclinical model of tissue expansion in Sprague-Dawley rats.
- To mimic physiological and pathological aspects of human tissue expansion.
- To provide a foundation for investigating regenerative mechanisms and improving clinical protocols.
Main Methods:
- A 10 mL tissue expander was implanted dorsally in rats.
- Initial saline injection of 5 mL followed by a two-week recovery period.
- Subsequent twice-weekly injections of 10 mL saline were administered.
Main Results:
- Tissue expansion demonstrated rapid initial skin growth, followed by deceleration.
- Late-stage complications included skin necrosis, tissue breakdown, and expander exposure.
- The model successfully replicated key aspects of clinical tissue expansion.
Conclusions:
- A reproducible preclinical tissue expansion model in rats was successfully established.
- This model serves as a foundation for studying skin regeneration and optimizing clinical protocols.
- The model allows for investigation into the mechanisms underlying tissue expansion and its associated complications.
