Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

8.8K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
8.8K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.7K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of heat shock protein 70 in oral epithelial dysplasia and squamous cell carcinoma.

Journal of cancer research and therapeutics·2024
Same author

Myofibroblasts in Odontogenic Cysts and Tumors: An Immunohistochemical Study.

Journal of microscopy and ultrastructure·2023
Same author

Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma.

Journal of oral and maxillofacial pathology : JOMFP·2022
Same author

Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma.

Journal of microscopy and ultrastructure·2020
Same author

Assessment of resistance to rice tungro disease in popular rice varieties in India by introgression of a transgene against Rice tungro bacilliform virus.

Archives of virology·2019
Same author

Hemangiomatous ameloblastoma: Case report with a brief review.

Journal of oral and maxillofacial pathology : JOMFP·2018

Related Experiment Video

Updated: Mar 25, 2026

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
06:04

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

Published on: September 16, 2022

6.7K

Dry Ice in Decoverslipping Microscopic Slides: A Novel Approach.

Soujanya Pinisetti1, Alivelu Dasarathi1, M Jyothsna1

  • 1Department of Oral Pathology, Government Dental College and Hospital, Kadapa, IND.

Cureus
|March 24, 2026
PubMed
Summary

Dry ice offers a faster, safer method for removing coverslips from histopathological slides compared to xylene. This technique preserves slide quality and reduces laboratory hazards.

Keywords:
decoverslippingdry icehistopathological slidesrestainingxylene

More Related Videos

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
08:52

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Published on: March 23, 2018

15.4K
Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope
08:53

Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope

Published on: December 19, 2025

2.9K

Related Experiment Videos

Last Updated: Mar 25, 2026

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
06:04

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

Published on: September 16, 2022

6.7K
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
08:52

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Published on: March 23, 2018

15.4K
Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope
08:53

Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope

Published on: December 19, 2025

2.9K

Area of Science:

  • Pathology
  • Histopathology
  • Laboratory Science

Background:

  • Histopathological slides are crucial for diagnosis but can fade over time.
  • Coverslip removal and restaining are necessary to restore slide quality.
  • Conventional xylene use is time-consuming and poses health risks.

Purpose of the Study:

  • To compare the effectiveness of dry ice versus xylene for coverslip removal from microscopic slides.
  • To evaluate time efficiency, tissue damage, and staining quality of both methods.

Main Methods:

  • A retrospective study involving 56 archival histopathological slides.
  • Random selection and categorization of slides into dry ice and xylene groups.
  • Measurement of coverslip removal time and assessment of tissue damage post-procedure.

Main Results:

  • Dry ice removal averaged 65 seconds, while xylene took approximately 4 days.
  • Minor, statistically insignificant tissue distortion was observed with dry ice (p = 0.075).
  • Both methods yielded comparable hematoxylin and eosin (H&E) staining results (p = 1.00).

Conclusions:

  • Dry ice is a significantly faster and effective alternative to xylene for coverslip removal.
  • This method minimizes tissue distortion and maintains staining quality.
  • Dry ice presents a safer, more environmentally friendly option for histopathology laboratories.