Fluorescence Quenching-Based Quantification of Famotidine in Pharmaceutical Formulations and Biological Fluids Using...

Area of Science:

• Analytical Chemistry
• Spectroscopy

Background:

• Famotidine is a widely used H2 receptor antagonist.
• Accurate quantification of famotidine is crucial for therapeutic efficacy and safety.
• Existing analytical methods may lack simplicity, selectivity, or economic viability.

Purpose of the Study:

• To develop a simple, selective, and economic spectrofluorimetric method for famotidine determination.
• To investigate the mechanism of fluorescence quenching by famotidine.
• To validate the method for analyzing famotidine in pharmaceutical and biological samples.

Main Methods:

• Spectrofluorimetric analysis utilizing Eosin Y as a fluorophore.
• Investigation of fluorescence quenching of Eosin Y by famotidine due to ion-pair complex formation.
• Optimization of reaction parameters and validation according to ICH guidelines.

Main Results:

• A linear correlation (R²=0.9969) was observed for famotidine concentrations from 1-20 μg/mL.
• The method demonstrated low limits of detection (4.67 ng/mL) and quantification (0.015 μg/mL).
• High percentage recoveries (98.78%-103.50%) were achieved in pharmaceutical and biological samples, with no interference from excipients.

Conclusions:

• The developed spectrofluorimetric method is effective, simple, selective, and economical for famotidine determination.
• The method is suitable for routine analysis of famotidine in pharmaceutical formulations and biological fluids.
• The analytical procedure exhibits good environmental sustainability.