Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 25, 2026

Production of Human CRISPR-Engineered CAR-T Cells
06:33

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

14.9K

Optimized High-Titer Lentivirus Production and Efficient CAR-T Cell Generation.

Atul Kumar Roy1,2, Ashwani Singh1,2, Jyotsna Dandotiya1,2

  • 1Centre for Immuno-biology and Immunotherapy, Translational Health Science and Technology Institute, Faridabad, Gurugram, India.

Current Protocols
|March 24, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kyasanur Forest Disease: Advances in Experimental Animal Models, Immune Assays, and Challenges in Vaccine Development.

Reviews in medical virology·2026
Same author

Corrigendum to "The cellular consequences of particulate matter pollutants in plants: Safeguarding the harmonious integration of structure and function" [Sci. Total Environ. 914 (2024), 169763].

The Science of the total environment·2026
Same author

Emerging roles of IL-9 and Th9 cells in respiratory viral illnesses.

Seminars in immunology·2026
Same author

Temperature derived morphological engineering in gold nanoparticles for colorimetric sensing of chromium ions in grape juice and tap water.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.

Human vaccines & immunotherapeutics·2026
Same author

Mesenchymal stem cells alleviate experimental cerebral malaria disease severity by inducing RoRγt<sup>+</sup> Foxp3<sup>+</sup> T regulatory (Tr 17) cells and modulating the dysregulated Th17/Treg axis.

Cell death discovery·2026

Manufacturing chimeric antigen receptor (CAR)-T cells is challenging. This study optimizes lentiviral vector (LV) production to enhance viral titers, enabling scalable, reproducible, and economical CAR-T cell generation for broader accessibility.

Area of Science:

  • Biotechnology
  • Immunotherapy
  • Cellular Therapy

Background:

  • Chimeric antigen receptor (CAR)-T cell therapy shows high efficacy in B-cell malignancies and emerging potential in autoimmune diseases.
  • Current CAR-T cell manufacturing is hindered by challenges in producing critical components like high-titer lentiviral vectors (LVs).
  • Lack of optimized protocols complicates CAR-T cell production, limiting accessibility.

Purpose of the Study:

  • To optimize lentiviral vector (LV) production for enhanced viral titers.
  • To develop a scalable, reproducible, and economical framework for CAR-T cell manufacturing.
  • To facilitate preclinical and translational research by streamlining CAR-T cell generation.

Main Methods:

  • Optimized LV production by screening plasmid ratios and enhancing transfection efficiency in HEK293T cells.
Keywords:
CAR‐T cellsadoptive cellular immunotherapylentivirustransductiontransfection

More Related Videos

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
16:04

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

15.1K
Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices
09:30

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Published on: December 5, 2025

650

Related Experiment Videos

Last Updated: Mar 25, 2026

Production of Human CRISPR-Engineered CAR-T Cells
06:33

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

14.9K
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
16:04

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

15.1K
Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices
09:30

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Published on: December 5, 2025

650
  • Fine-tuned peripheral blood mononuclear cell (PBMC) activation and transduction conditions.
  • Developed protocols for scalable production of third-generation LVs and CAR-T cell generation.

    • Main Results:

    • Achieved enhanced viral titers through optimized LV production methods.
    • Established a reproducible and economical process for generating CAR-T cells.
    • Provided a framework for efficient CAR-T cell manufacturing.

    Conclusions:

    • Optimized LV production protocols significantly improve CAR-T cell manufacturing.
    • The developed methods facilitate scalable, reproducible, and cost-effective CAR-T cell generation.
    • This work supports broader accessibility and application of CAR-T cell therapies.