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Updated: Mar 25, 2026
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Cyclin B3 dsRNA Orchestrate Meiotic Progression in Porcine Oocytes
Yanlong Zhu1,2, Xi-Qing Jiang1,2, Shuang Gao1,2
1Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.
Journal of Molecular Cell Biology
|March 24, 2026
Summary
Cyclin B3 (CCNB3) is crucial for pig oocyte meiosis. Its stability is regulated by CCNB3-AS RNA and Vimentin, impacting meiotic progression and revealing new roles in mRNA degradation.
Area of Science:
- Reproductive Biology
- Molecular Cell Biology
- RNA Biology
Background:
- Cyclin B3 (CCNB3) is vital for mammalian meiotic progression.
- Its specific functions in porcine oocytes are not well understood.
- CCNB3 interacts with CDK1 to regulate MPF activity.
Purpose of the Study:
- To elucidate the functional mechanisms of CCNB3 in porcine oocytes.
- To identify regulatory factors controlling CCNB3 mRNA stability.
- To understand the role of CCNB3-AS and Vimentin in oocyte meiosis.
Main Methods:
- Knockdown of CCNB3 in porcine oocytes.
- Analysis of cyclin B1 and securin degradation.
- RNA-RNA interaction studies (CCNB3-AS and CCNB3 mRNA).
- Protein-RNA interaction studies (CCNB3-AS, Vimentin, PATL1, CNOT7).
- Structural analysis of protein interactions.
Main Results:
- CCNB3 knockdown caused meiotic arrest at metaphase I.
- Impaired degradation of cyclin B1 and securin was observed.
- CCNB3-AS forms a dsRNA with CCNB3 mRNA, enhancing stability against PATL1-mediated degradation.
- Vimentin interacts with CCNB3-AS and influences PATL1-CNOT7 binding, stabilizing CCNB3 mRNA.
- Vimentin plays a non-canonical role in mRNA degradation.
Conclusions:
- CCNB3-AS/CCNB3 dsRNA duplex, Vimentin, and PATL1 collectively regulate porcine oocyte meiosis.
- Vimentin's novel role in mRNA degradation provides insights into meiotic regulation.
- This study offers new theoretical support for understanding porcine oocyte meiosis.
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