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Updated: Mar 25, 2026

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Cyclin B3 dsRNA Orchestrate Meiotic Progression in Porcine Oocytes

Yanlong Zhu1,2, Xi-Qing Jiang1,2, Shuang Gao1,2

  • 1Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.

Journal of Molecular Cell Biology
|March 24, 2026
PubMed

Insights

Cyclin B3 (CCNB3) is crucial for pig oocyte meiosis. Its stability is regulated by CCNB3-AS RNA and Vimentin, impacting meiotic progression and revealing new roles in mRNA degradation.

Area of Science:

  • Reproductive Biology
  • Molecular Cell Biology
  • RNA Biology

Background:

  • Cyclin B3 (CCNB3) is vital for mammalian meiotic progression.
  • Its specific functions in porcine oocytes are not well understood.
  • CCNB3 interacts with CDK1 to regulate MPF activity.

Purpose of the Study:

  • To elucidate the functional mechanisms of CCNB3 in porcine oocytes.
  • To identify regulatory factors controlling CCNB3 mRNA stability.
  • To understand the role of CCNB3-AS and Vimentin in oocyte meiosis.

Main Methods:

  • Knockdown of CCNB3 in porcine oocytes.
  • Analysis of cyclin B1 and securin degradation.
  • RNA-RNA interaction studies (CCNB3-AS and CCNB3 mRNA).
  • Protein-RNA interaction studies (CCNB3-AS, Vimentin, PATL1, CNOT7).
  • Structural analysis of protein interactions.

Main Results:

  • CCNB3 knockdown caused meiotic arrest at metaphase I.
  • Impaired degradation of cyclin B1 and securin was observed.
  • CCNB3-AS forms a dsRNA with CCNB3 mRNA, enhancing stability against PATL1-mediated degradation.
  • Vimentin interacts with CCNB3-AS and influences PATL1-CNOT7 binding, stabilizing CCNB3 mRNA.
  • Vimentin plays a non-canonical role in mRNA degradation.

Conclusions:

  • CCNB3-AS/CCNB3 dsRNA duplex, Vimentin, and PATL1 collectively regulate porcine oocyte meiosis.
  • Vimentin's novel role in mRNA degradation provides insights into meiotic regulation.
  • This study offers new theoretical support for understanding porcine oocyte meiosis.
Keywords:
RNA stabilitycyclin B3lncRNAoocyte maturationpig

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