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Related Experiment Video

Updated: Mar 27, 2026

Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

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Spoti-find: a novel, open-source void spot assay image analysis tool.

Cara C Hardy1, Susan Sheehan1, Seamus Mawe1

  • 1The Jackson Laboratory, Bar Harbor, Maine, United States.

American Journal of Physiology. Renal Physiology
|March 24, 2026
PubMed
Summary
This summary is machine-generated.

Spoti-find is a new, open-source software for analyzing rodent void spot assays (VSA). It offers user-friendly tools and novel metrics to better understand urinary behavior in preclinical studies.

Keywords:
bladdermousevoid spot assay

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Area of Science:

  • Preclinical research
  • Urology
  • Animal behavior

Background:

  • The void spot assay (VSA) is crucial for studying rodent urinary behavior.
  • Current VSA analysis tools lack scope, throughput, or accessibility.

Purpose of the Study:

  • To develop Spoti-find, an open-source, user-friendly VSA image analysis application.
  • To introduce novel metrics for enhanced VSA data interpretation.

Main Methods:

  • Developed Spoti-find, a stand-alone application with a graphical interface.
  • Implemented manual/semi-automated identification, adjustable thresholds, and data export.
  • Introduced metrics: void circularity, distance to edge, and volume-based void binning.

Main Results:

  • Spoti-find demonstrated strong inter-user consistency and sensitivity to phenotypes.
  • The software accurately captured novel parameters with high agreement to existing tools.
  • Validated across diverse datasets, including aging and disease models.

Conclusions:

  • Spoti-find enhances the interpretive power of VSA by capturing behavioral context and spatial morphology.
  • Provides a flexible, user-friendly platform for phenotyping urinary dysfunction in research.
  • Expands VSA capabilities for preclinical studies on urinary disorders.