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Updated: Mar 27, 2026

Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products
Published on: December 5, 2020
Natural products and biological activity from fungal symbionts associated with Rosmarinus officinalis L.
Xiaoyan Hong1, Hui Zhang2, Shengli Yang1
1The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
Abstract:
An endophytic fungus isolated from the rhizomes of Rosmarinus officinalis was identified as a Talaromyces species. Five distinct compounds were successfully isolated from the crude extract obtained from the fermentation culture, and identified as (1) thymol, (2) phloretin, (3) hesperetin, (4) dihydroquercetin, and (5) 6,7-dihydroxycoumarin. The antimicrobial activities of the five compounds were evaluated against Candida albicans, Escherichia coli, and Staphylococcus aureus, revealing varied efficacy. Compound 2 was the most effective inhibitor of C. albicans. Activity against E. coli was observed for compounds 1, 2, 3, and 5. In the hydroxyl radical (·OH) scavenging assay, all five compounds exhibited weak reducing capacity. In the DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays, compound 5 exhibited the highest scavenging activity. Additionally, cytotoxicity assays revealed that among the ID8, HeLa, and 293 T cell lines tested, the five compounds exhibited the strongest inhibitory effects against the ID8 cell line.
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