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Updated: Mar 27, 2026
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Coagulation Factors in Mexican Children, Young Adults and Older Adults
Jessica Viviana Nieves-Hernández1, Edgar Hernández-Zamora2, Marlene Galicia-Alvarado3
1Morphology Department, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (ENCB, IPN), Mexico City, Mexico.
Abstract:
BackgroundThe diagnosis of bleeding disorders through the Clinical Chemistry Laboratory requires specific tests. In Mexico, there are few reports on reference values (RVs) for coagulation factors (CFs). The prevalence and RVs in adults and older Mexican adults have already been described. However, it remains unknown whether these parameters differ among minors (MI), young adults (YA), and older adults (OA). The aim of this study was to determine CF levels in a group of healthy MI, YA, and OA in Mexico.MethodsCFs were determined on a coagulation analyzer using commercial kits for the CFs: I, II, V, VII, VIII, IX, X, XI, and XII. The effect size was determined using Glass's rank biserial correlation coefficient. Comparisons between groups were performed using Dunn-Bonferroni post hoc tests following a significant Kruskal-Wallis test, and the epsilon-squared coefficient was used to estimate effect magnitude.Results347 participants, stratified by age group and sex, were studied. Variability in CF activity was observed. Age was found to be an important factor influencing the activity of all CFs, particularly factors II, V, VIII, IX, X, and XII, with factors V and XII proposed as biomarkers of aging.ConclusionsCF levels observed in OA differ from those in YA and MI, as well as from the RVs proposed in commercial kits. It is important to integrate these results into established diagnostic algorithms, as well as into an evidence-based practical guide that clinical personnel can use to support a comprehensive and accurate diagnosis of patients with hemostatic disorders.
Insights
Coagulation factor levels in Mexican adults vary significantly by age, with factors V and XII potentially serving as aging biomarkers. These findings necessitate updated diagnostic reference values for accurate bleeding disorder diagnosis.
Area of Science:
- Clinical Chemistry
- Hematology
- Gerontology
Background:
- Accurate diagnosis of bleeding disorders relies on clinical chemistry laboratory tests.
- Limited data exists on reference values for coagulation factors in Mexico.
- Previous studies focused on adults and older adults, leaving pediatric and young adult ranges undefined.
Purpose of the Study:
- To determine coagulation factor levels in healthy Mexican minors, young adults, and older adults.
- To investigate age-related differences in coagulation factor activity.
- To establish age-specific reference values for coagulation factors in Mexico.
Main Methods:
- Coagulation analyzer with commercial kits used to measure coagulation factors I, II, V, VII, VIII, IX, X, XI, and XII.
- Statistical analysis included Kruskal-Wallis tests, Dunn-Bonferroni post hoc tests, and effect size calculations (Glass's rank biserial correlation, epsilon-squared).
- 347 participants were stratified by age group (minors, young adults, older adults) and sex.
Main Results:
- Significant variability in coagulation factor activity was observed across age groups.
- Age was a significant factor influencing the activity of all tested coagulation factors.
- Factors V and XII showed notable age-related changes, suggesting their potential as biomarkers of aging.
Conclusions:
- Coagulation factor levels in older adults differ from those in younger age groups and established commercial kit reference values.
- These findings underscore the need for age-specific reference values in Mexico.
- Integration into diagnostic algorithms and clinical guidelines is crucial for accurate hemostatic disorder diagnosis.
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