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Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging
Published on: January 19, 2024
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Structure and function of mouse lens suture examined by 2-photon fluorescence microscopic imaging
Qinrong Zhang1,2, Jun Zhu3, Taishi Painter4,5
1Department of Physics, University of California, Berkeley, CA, 94720, USA.
Scientific Reports
|March 25, 2026
Summary
Klotho-like protein homology knockout mice develop suture cataracts due to disorganized lens sutures. This study reveals morphological changes in sutures and fiber pathology, identifying new markers for cataracts.
Area of Science:
- Ophthalmology
- Cell Biology
- Biophysics
Background:
- Lens sutures are crucial for microcirculation and transparency.
- Klotho-like protein homology (KLPH) knockout (KLPH-KO) mice develop suture cataracts.
- KLPH is a type I membrane glycoprotein involved in lens integrity.
Purpose of the Study:
- To investigate lens fiber and suture architecture and permeability in KLPH-KO mice.
- To identify morphological markers for suture cataracts.
- To elucidate the role of KLPH in maintaining lens homeostasis.
Main Methods:
- Two-photon fluorescence microscopy (2PFM) for in vivo imaging.
- 3D imaging to analyze suture geometries and variations.
- Structural Similarity Index (SSIM) to quantify pattern randomization.
Main Results:
- KLPH-KO lenses showed heterogeneous suture morphologies and misalignment.
- Increased pattern randomization in KLPH-KO sutures (p < 0.05).
- Voids and enlarged vacuoles observed in both WT and KLPH-KO lenses; KLPH-KO lenses had irregular central voids with amorphous structures.
Conclusions:
- Novel morphological markers for suture cataracts and fiber pathology were identified.
- Lens sutures act as stabilized interfaces, maintaining lens integrity and fiber organization.
- KLPH plays a critical role in lens suture morphology and transparency.
