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  1. Home
  3. Microfluidic Fluorescence-activated Cell Sorting Via Gradient Dielectrophoresis.
  1. Home
  3. Microfluidic Fluorescence-activated Cell Sorting Via Gradient Dielectrophoresis.

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Microfluidic Fluorescence-Activated Cell Sorting via Gradient Dielectrophoresis.

Yating Hu1,2, Junchi Song3, Yuchen Li4

  • 1Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, Liaoning Province 110819, China.

Analytical Chemistry
|March 25, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

We developed a compact microfluidic system using gradient dielectrophoresis for cell sorting. This portable device offers gentle, accurate sorting of small samples, enabling on-chip cytometry and point-of-care testing.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Sorting Technology

Background:

  • Conventional fluorescence-activated cell sorting (FACS) systems are costly and require large sample volumes.
  • There is a need for portable, low-volume cell sorting solutions for advanced applications.
  • Microfluidic devices offer potential for miniaturized and efficient cell manipulation.

Purpose of the Study:

  • To develop a compact, integrated microfluidic system for cell sorting.
  • To utilize gradient dielectrophoresis (gDEP) for precise cell manipulation on-chip.
  • To demonstrate the system's capability for sorting fluorescent particles and cancer cells.

Main Methods:

  • Integration of inertial self-ordering, laser-induced fluorescence detection, and programmable cell deflection on a single chip.
  • Utilizing asymmetric curved microchannels for uniform single-cell distribution.
  • Employing curved gradient electrodes with intermittent sinusoidal signals for controlled cell sorting and prevention of electrode adhesion.

    • Main Results:

    • Efficient screening of fluorescent microspheres.
    • High-viability isolation of human cancer cells expressing green fluorescent protein (GFP).
    • Demonstration of a portable system for gentle and accurate sorting of small sample volumes.

    Conclusions:

    • The developed gradient dielectrophoresis microfluidic fluorescence-activated cell sorting (gDEP-μFACS) system is a compact and efficient alternative to conventional FACS.
    • The portable design facilitates on-chip cytometry and point-of-care testing applications.
    • The system achieves high cell viability and sorting accuracy for various biological samples.